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plant expression vector pmdc32 hpb  (Addgene inc)


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    Structured Review

    Addgene inc plant expression vector pmdc32 hpb
    Plant Expression Vector Pmdc32 Hpb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmdc32-hpb/pMDC32-HPB+(Plasmid+%2332078)/bio_rxiv__2025__05__06__652549-219-13-18
    Average 93 stars, based on 13 article reviews
    plant expression vector pmdc32 hpb - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation
    Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches.

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape
    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Article Title: The Regulatory-associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis
    Article Snippet: For Pro35S::6xMyc-RAPTOR1B construct, previously cloned 6xMyc-RAPTOR1B in 179 pE3n for the complementation lines in A. thaliana (see above) was subcloned via Gateway into the plant 180 destination vector pMDC32-HPB (Addgene, https://www.addgene.org), which harbors a 2X CaMV 35S 181 promoter upstream of the insertion cassette to generate Pro35S::6XMyc-RAPTOR1B.

    Clone Assay:

    Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation
    Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches.

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape
    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Article Title: The Regulatory-associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis
    Article Snippet: For Pro35S::6xMyc-RAPTOR1B construct, previously cloned 6xMyc-RAPTOR1B in 179 pE3n for the complementation lines in A. thaliana (see above) was subcloned via Gateway into the plant 180 destination vector pMDC32-HPB (Addgene, https://www.addgene.org), which harbors a 2X CaMV 35S 181 promoter upstream of the insertion cassette to generate Pro35S::6XMyc-RAPTOR1B.

    Plasmid Preparation:

    Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation
    Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches.

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape
    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Article Title: The Regulatory-associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis
    Article Snippet: For Pro35S::6xMyc-RAPTOR1B construct, previously cloned 6xMyc-RAPTOR1B in 179 pE3n for the complementation lines in A. thaliana (see above) was subcloned via Gateway into the plant 180 destination vector pMDC32-HPB (Addgene, https://www.addgene.org), which harbors a 2X CaMV 35S 181 promoter upstream of the insertion cassette to generate Pro35S::6XMyc-RAPTOR1B.

    Sequencing:

    Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation
    Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches.

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape
    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Article Title: The Regulatory-associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis
    Article Snippet: For Pro35S::6xMyc-RAPTOR1B construct, previously cloned 6xMyc-RAPTOR1B in 179 pE3n for the complementation lines in A. thaliana (see above) was subcloned via Gateway into the plant 180 destination vector pMDC32-HPB (Addgene, https://www.addgene.org), which harbors a 2X CaMV 35S 181 promoter upstream of the insertion cassette to generate Pro35S::6XMyc-RAPTOR1B.

    Recombinant:

    Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation
    Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches.

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape
    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Article Title: The Regulatory-associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis
    Article Snippet: For Pro35S::6xMyc-RAPTOR1B construct, previously cloned 6xMyc-RAPTOR1B in 179 pE3n for the complementation lines in A. thaliana (see above) was subcloned via Gateway into the plant 180 destination vector pMDC32-HPB (Addgene, https://www.addgene.org), which harbors a 2X CaMV 35S 181 promoter upstream of the insertion cassette to generate Pro35S::6XMyc-RAPTOR1B.

    Derivative Assay:

    Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation
    Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches.

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape
    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Article Title: The Regulatory-associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis
    Article Snippet: For Pro35S::6xMyc-RAPTOR1B construct, previously cloned 6xMyc-RAPTOR1B in 179 pE3n for the complementation lines in A. thaliana (see above) was subcloned via Gateway into the plant 180 destination vector pMDC32-HPB (Addgene, https://www.addgene.org), which harbors a 2X CaMV 35S 181 promoter upstream of the insertion cassette to generate Pro35S::6XMyc-RAPTOR1B.

    Fluorescence:

    Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation
    Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches.

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape
    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Article Title: The Regulatory-associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis
    Article Snippet: For Pro35S::6xMyc-RAPTOR1B construct, previously cloned 6xMyc-RAPTOR1B in 179 pE3n for the complementation lines in A. thaliana (see above) was subcloned via Gateway into the plant 180 destination vector pMDC32-HPB (Addgene, https://www.addgene.org), which harbors a 2X CaMV 35S 181 promoter upstream of the insertion cassette to generate Pro35S::6XMyc-RAPTOR1B.

    Amplification:

    Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation
    Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches.

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape
    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Article Title: The Regulatory-associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis
    Article Snippet: For Pro35S::6xMyc-RAPTOR1B construct, previously cloned 6xMyc-RAPTOR1B in 179 pE3n for the complementation lines in A. thaliana (see above) was subcloned via Gateway into the plant 180 destination vector pMDC32-HPB (Addgene, https://www.addgene.org), which harbors a 2X CaMV 35S 181 promoter upstream of the insertion cassette to generate Pro35S::6XMyc-RAPTOR1B.

    Expressing:

    Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation
    Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches.

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape
    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Article Title: The Regulatory-associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis
    Article Snippet: For Pro35S::6xMyc-RAPTOR1B construct, previously cloned 6xMyc-RAPTOR1B in 179 pE3n for the complementation lines in A. thaliana (see above) was subcloned via Gateway into the plant 180 destination vector pMDC32-HPB (Addgene, https://www.addgene.org), which harbors a 2X CaMV 35S 181 promoter upstream of the insertion cassette to generate Pro35S::6XMyc-RAPTOR1B.



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    Image Search Results


    Gene structure and amino acid sequence alignment analysis of VvCYP76F14 proteins. ( A ) Gene structure of VvCYP76F14 s derived from three mutant lines. ( B ) Acid sequence alignment of VvCYP76F14 proteins.

    Journal: Genes

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape

    doi: 10.3390/genes15111478

    Figure Lengend Snippet: Gene structure and amino acid sequence alignment analysis of VvCYP76F14 proteins. ( A ) Gene structure of VvCYP76F14 s derived from three mutant lines. ( B ) Acid sequence alignment of VvCYP76F14 proteins.

    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Techniques: Sequencing, Derivative Assay, Mutagenesis

    Subcellular localization of VvCYP76F14. The CDS of VvCYP76F14 derived from ‘Yantai 2-2-08’ and ‘Yantai 2-2-08’ and further cloned into the pBWA(V)HS-ccdb-GLosgfp vector. The GV3101 strain harboring the pBWA(V)HS-CYP76F14-Glosgfp or the empty vector was infiltrated into Arabidopsis mesophyll protoplasts. The GFP fluorescence and chloroplast autofluorescence were observed using the excitation/emission wavelengths 470/510 nm and 620/660 nm, respectively. Scale bar = 10 μm.

    Journal: Genes

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape

    doi: 10.3390/genes15111478

    Figure Lengend Snippet: Subcellular localization of VvCYP76F14. The CDS of VvCYP76F14 derived from ‘Yantai 2-2-08’ and ‘Yantai 2-2-08’ and further cloned into the pBWA(V)HS-ccdb-GLosgfp vector. The GV3101 strain harboring the pBWA(V)HS-CYP76F14-Glosgfp or the empty vector was infiltrated into Arabidopsis mesophyll protoplasts. The GFP fluorescence and chloroplast autofluorescence were observed using the excitation/emission wavelengths 470/510 nm and 620/660 nm, respectively. Scale bar = 10 μm.

    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Techniques: Derivative Assay, Clone Assay, Plasmid Preparation, Fluorescence

    In vitro activity analysis of VvCYP76F14 in Escherichia coli . The in vitro enzymatic activity of VvCYP76F14 was assayed by measuring the amount of remnant substrate. Data are shown as means ± SE ( n = 3). Letters indicate significant differences among five VvCYP76F14s at a significance level of p ≤ 0.05, as determined using ANOVA followed by Fisher’s LSD test.

    Journal: Genes

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape

    doi: 10.3390/genes15111478

    Figure Lengend Snippet: In vitro activity analysis of VvCYP76F14 in Escherichia coli . The in vitro enzymatic activity of VvCYP76F14 was assayed by measuring the amount of remnant substrate. Data are shown as means ± SE ( n = 3). Letters indicate significant differences among five VvCYP76F14s at a significance level of p ≤ 0.05, as determined using ANOVA followed by Fisher’s LSD test.

    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Techniques: In Vitro, Activity Assay

    Transient expression of VvCYP76F14 in berries of D299T mutant lines. The content levels of linalool, ( E )-8-hydroxylinalool, ( E )-8-oxolinalool, and ( E )-8-carboxylinalool in berries of ‘Yantai 2-2-08’ ( A ), ‘Yantai 2-2-19’ ( B ), and ‘Yantai 2-3-37’ ( C ) were assayed by UPLC-MS. Data are shown as means ± SE ( n = 3). Letters indicate significant differences among five VvCYP76F14s at a significance level of p ≤ 0.05, as determined using ANOVA followed by Fisher’s LSD test.

    Journal: Genes

    Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape

    doi: 10.3390/genes15111478

    Figure Lengend Snippet: Transient expression of VvCYP76F14 in berries of D299T mutant lines. The content levels of linalool, ( E )-8-hydroxylinalool, ( E )-8-oxolinalool, and ( E )-8-carboxylinalool in berries of ‘Yantai 2-2-08’ ( A ), ‘Yantai 2-2-19’ ( B ), and ‘Yantai 2-3-37’ ( C ) were assayed by UPLC-MS. Data are shown as means ± SE ( n = 3). Letters indicate significant differences among five VvCYP76F14s at a significance level of p ≤ 0.05, as determined using ANOVA followed by Fisher’s LSD test.

    Article Snippet: Sequencing-verified VvCYP76F14 was cloned into the pMDC32-HPB (Addgene: 32078) to generate the recombinant plasmid pMDC32-HPB- CYP76F14 .

    Techniques: Expressing, Mutagenesis